![]() ![]() ![]() (2022–2023) ID UJ Individual Research Grant, POB BioS. Sławomir Lasota: Migratory and electrotactile potential of fibroblast microenvironment of solid pancreatic tumor.Genetic modification of cells using lentiviral vectors transfections by chemical methods and nucleofection using the Neon Transfection System (Invitrogen).Cell imaging under hypoxic conditions using the JuLiStage system (NanoEntek).Isolation of nanoparticles from biological fluids (Optima XPN Ultracentrifuge, Beckman Coulter)Cell cultures under low oxygen conditions (hypoxia) in the InvivO2 chamber (Russkin) models of hypoxia and reoxygenation.High-resolution flow cytometry dedicated for nanoparticle analysis of (Apogee A60-Micro-PLUS, Apogee Flow Systems).Imaging flow cytometry (ImageStreamX Mark II Imaging Flow Cytometer, Luminex Corp.).Classical flow cytometry (Guava Eas圜yte, Merck).Measurement of nanoparticles concentration and size by Nanoparticle Tracking Analysis (NTA) (Nanosight NS300, Malvern).Neon Electroporation System (Invitrogen) for cell electroporation.Olympus IX81 fluorescent microscope equiped with MicroPublisher 3.3 RTV camera.FACSAria III (Becton Dickinson) fluorescence-activated cell sorting system.CFX384 Real Time System (BioRad) for gene expression analysis on 384 well plates.Water purification system Simplicity UV (Merk).Development of novel stem cell- and EV-based advanced therapy medicinal products (ATMPs) for their use in treatment of patients with civilization diseases (preclinical and clinical studies).Optimization of biocompatible scaffolds combining biomaterials (including graphene) and defined stem cell populations for tissue regeneration.Role of stem cell derived extracellular vesicles (native and genetically modified) in tissue.Optimization of procedures for various stem cell and EV fractions identification and isolation for their use in regenerative medicine.Applications of stem cells and stem cell derived extracellular vesicles (EVs) in regenerative medicine.Optimization of stem cell and progenitor cell populations for applications in experimental cardiology and orthopedy including induced pluripotent stem cells (iPSCs) as well as mesenchymal stem cells (MSCs) of different antigenic phenotype various types. ![]()
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